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KMID : 1234420100380040455
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 4 p.455 ~ p.460
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Application of 16S rDNA PCR-RFLP Analysis for the Rapid Identification of Weissella Species
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Lee Myong-Jae
Cho Kyeung-Hee
Lee Jong-Hoon
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Abstract
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A polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis was applied to detect and identify ten Weissella spp. frequently found in kimchi. The previously reported genus-specific primers designed from 16S rDNA sequences of Weissella spp. were adopted but PCR was performed at the increased annealing temperature by 4oC. The sizes of amplified PCR products and restricted fragments produced by AluI, MseI, and BceAI endonucleases were well correspond with the expected sizes. W. kandleri, W. koreensis, W. confusa, W. minor, W. viridescens, W. cibaria, and W. soli were distinguished by AluI and MseI and W. hellenica and W. paramesenteroides were identified by BceAI. W. thailandensis was distinguished when restriction pattern of other species was compared but identified by the single use of MspI.
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KEYWORD
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16S rDNA, RCR-RFLP, Weissella, kimchi
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